FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373368
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCATA1551.8189894E-1229.3573441
AGCATAC1606.548362E-1126.252242
CTATAGG1401.1903467E-825.0021321
CATACGT2100.025.0021324
ATCTTTT16550.023.0499697
TACGTCT1905.638867E-1022.105546
CCTAGGA1755.2496034E-922.0018772
TCTTTTT16550.021.9926328
CTTTTTT16800.021.665369
GATCTTT20100.021.592316
TTAGAAT3900.020.6427883
CAGGGGT3400.020.5899934
ATAGCAT1055.464163E-420.0017053
TTTATTC1055.464163E-420.0017053
TAGGATC1751.2648161E-720.0017054
GTACAAA1601.1857865E-619.6891781
ACTATAT1257.8226476E-519.6016731
ACCGTTA7150.019.5792398
TAGTACA900.00527477619.4461024
TAGAATT4000.019.2516424
Produced by FastQC (version 0.11.5)