FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821025
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT43850.026.0174318
ATCTTTT44500.025.7946857
GATCTTT49100.025.1627066
CTTTTTT45400.024.9750049
GTCTAGG3500.023.0010551
TTTCGGA6750.022.8158631
CGTGTGG18500.022.70686170
TAACATT850.003791800520.5880494
CATTACT2453.092282E-1119.999822
ATTACTC2302.8376235E-1019.782433
TATGTAC900.00527830119.444273
TTCGGAA7750.019.419182
TCATTAC3250.019.3855041
GTATCAT1107.4771064E-419.0917851
TATATAG1107.4798695E-419.0907365
GTGTTAT2405.0386006E-1018.9560862
TTACCTA2607.2759576E-1118.84960770
CTATAAG1301.0576956E-418.847021
TGTACAC950.007212555518.4208853
TCTAGGG6500.018.3075282
Produced by FastQC (version 0.11.5)