FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417479
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATA1001.39852655E-524.5012112
ATCTTTT34950.024.4326657
GATCTTT38600.024.2093326
TCTTTTT35250.023.728328
TTTCGGA5800.023.5356461
CTTTTTT35600.023.4950379
TTTACTC1652.6630005E-923.3336623
TATAGGT2401.8189894E-1221.8753075
GACTCAT800.002668871321.87299312
GTATACG1807.2686817E-921.3899461
TTCGGAA6750.021.260312
TATGGTA2001.0713848E-920.99807411
TCTAGGG5500.020.3646432
TTTATAG2453.092282E-1120.0002823
CGTGTGG16050.019.84801770
CTCTAGG1951.8260835E-819.7445661
CGCTAGT1601.1846441E-619.69125269
AATCGTA1801.7013917E-719.4467846
TAACTAA1801.702665E-719.44540638
CTAGGGG4350.019.3106173
Produced by FastQC (version 0.11.5)