FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402197
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG53250.028.393995
TTCCGAT138300.027.4853292
TATATAC1056.779228E-726.6663133
CCGATCT145500.025.9068744
TCCGATC149850.025.4818123
ATCTGAG6450.024.4165387
TACTATA1301.607259E-724.2321762
GATCTTT31850.023.7359496
CTTCCGA161700.023.7026771
CGATCTA25400.023.5626815
ATCTTTT31950.023.11228812
GATCTGA15750.023.1108066
TCTTTTT32800.022.9409513
GATCTGG30600.022.7611356
GATCTAG9850.022.0301656
ATCTAGG4300.021.9748867
CTTTTTT33850.021.91994714
ACGTTAC800.00266653721.876271
CGATCTT51950.021.222055
GTATAAT1656.798291E-821.2133541
Produced by FastQC (version 0.11.5)