FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014363
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT21560.1070313543288871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG1301.6090598E-724.2304861
ATCTTTT36350.023.3955087
TTAGTAT1802.8740033E-1023.3319023
CGTGTGG15700.022.29494770
GATCTTT42800.022.0780856
CTTTTTT39250.021.4885949
TCTTTTT39200.021.0696138
GTATTAT1751.2668352E-719.9997671
CTATAGG2757.2759576E-1219.0906851
ATTATAG4100.018.7802681
GTATTGA2508.6765795E-1018.199791
GATCTGG49450.017.9766526
TAGTATT2155.6021236E-817.9058784
AACGTAT3153.6379788E-1217.77668819
GTGTAAA3400.017.4997961
TATACTT2401.0315489E-817.4989285
TAGTACA1401.8674326E-417.4989264
CGATCTT92900.017.2163835
TGTCTCC8800.017.10377170
CACGAAT12150.016.99906244
Produced by FastQC (version 0.11.5)