FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269975
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC21780.17149943896533396Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC20880.16441268528908048No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA19500.1535463296521585Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC16910.1331522274060513No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC13750.10826984783165022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA5450.025.6883342
TTTCGGA4950.025.4538021
GTCATAA1151.4984162E-624.3471151
ATCTTTT47700.023.9930087
TAGTACA750.001833448423.332654
TCTTTTT49150.023.0003458
GATCTTT53050.022.760886
CTTTTTT49950.022.7011439
TACGTGT2650.022.45482468
TAGGGGT4150.021.927074
TTATACT1304.3466043E-621.5378324
GTATATA1154.120876E-521.3037241
CGTGTGG18700.020.9644370
GGGATAC4100.020.486464
TGCATCG10300.020.39016370
CATACGT2203.5051926E-919.0903514
GTATGTT1301.057985E-418.84560465
CTGGGGT4900.018.5708854
CTAGGGC1702.120727E-618.528873
GACTACG60800.018.3651270
Produced by FastQC (version 0.11.5)