FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790670
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC27110.15139584624749397No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC23670.13218515974467657No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC22410.12514868736283066Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC21930.1224681264554608No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA21380.11939665041576616Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT20150.11252771309063088No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC19600.10945623705093624No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT18580.10376004512277527No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA18550.10359251006606465No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT18400.10275483478251159No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC18360.10253145470689741No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG18130.10124701927211603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC3000.022.1650128
TCTTTTT35250.022.0409098
GCTAATG4150.021.93036370
TTTCGGA6600.021.2135011
CTTTTTT36500.021.19029
GTACTGG6800.021.1043131
TAATACT11850.020.970074
TACTAAT11850.020.9688997
TTCGGAA7200.020.4162852
ATCTTTT40450.020.2457087
AATCTAG1055.4657995E-420.00131
TAGTACT8600.019.9414844
GATCTTT45950.019.8037646
CGTAATA12200.019.794712
TATGCTT6050.019.6690525
TAATGCC12350.019.55319810
GAATCTA1801.7055027E-719.44299529
TACTGTA900.005280269319.4429957
TAAGACA2353.8016879E-1019.3613384
CATGTTA4900.019.28481521
Produced by FastQC (version 0.11.5)