FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295579
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.1102981755647475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT604.983222E-429.1680832
TTTCGGA5900.028.4759581
TTATACG657.957629E-426.9243834
ATCTGTG8700.024.9418147
ATCTTTT49250.024.0196177
TTCGGAA7000.024.0011652
TCTTTTT49750.023.5671638
GATCTTT53800.023.4853636
GGTATAA1202.169838E-623.33266464
GTATTGG2300.022.8271941
CTTTTTT52650.022.2690689
TGCATCG13150.022.09147570
CGTGTGG13700.021.9710270
CCTATAG800.002666577521.876062
CTATAGG1154.1184892E-521.3053821
TCGTACA1154.1184892E-521.3053822
TCTAGGC2950.020.1689138
AGCATAC1751.2651617E-720.000972
CATACGT1751.2651617E-720.000974
ACTAACA1055.465287E-420.000972
Produced by FastQC (version 0.11.5)