FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945133
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC29190.1500668591813516No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT28180.1448744121867245No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA27750.14266376643653672No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT26430.13587759808712307No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT25170.12939989193541007No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC23980.1232820583476811No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG22910.1177811491553534No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA21290.10945266981743666No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA20320.1044658642879433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT38250.024.4290227
TTTCGGA6000.023.9186691
TATACTA1202.1705873E-623.3328885
TCTTTTT40850.022.8741768
CTTTTTT40850.022.4458229
ACTAGAC1454.6117202E-721.7242813
TAGGGGT4400.021.4774174
ACCGGTA5600.021.24959415
TGCATCG12700.020.94717470
CAGGGGT4750.020.6317164
AGGACTA11350.020.3520365
TAGGACT11550.020.0001324
AGTTAGA12950.019.9975661
GTTAGAG13050.019.84432462
CTAGGAC12100.019.6695523
CATTAAT13600.019.5634844
AGGTTCG12550.019.52254567
GATCTTT50950.019.509456
AGAGGTT12900.019.26319765
TTCGGAA7300.019.1791952
Produced by FastQC (version 0.11.5)