FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334875
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA56330.24125488516515875No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC56190.2406552813319771No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT53030.22712136624016277No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT51920.22236736441993682No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC45930.19671288612880777No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG43600.18673376519085605No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA39790.17041597515926976No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA39100.16746078483858878No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA36530.15645377161518284No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT36140.15478344665131966No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG32830.14060709888109643No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA32800.14047861234541464No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC31760.13602441244177954No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATG26470.1133679533165587No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT25330.10848546496065099No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA24430.10463086889019756No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG24070.10308903046201616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6250.025.7614751
GGTCCAA21600.021.54846811
TCTTTTT41050.021.3139513
TAAGAGT3000.020.9994034
TGCATCG18350.020.98347570
CTAACAC3100.020.3220043
CTTTTTT43300.020.20641114
TAGGGGT5500.019.7267114
AGGACTA15850.019.6524375
CAAGAAT24200.019.52340315
GTCCTAG10900.018.9460371
CTCGCGT24500.018.85579929
CGTGTGG15750.018.66893470
CTCTCGC24750.018.66533727
CGCGTAA9400.018.61489721
ATCTGGG18350.018.4992527
AGAATTT25450.018.42697717
GATCTGG44350.018.1505576
CGTCGTA49250.018.12286433
AAGAATT26100.018.10215816
Produced by FastQC (version 0.11.5)