FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652043
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT8010.12284465901788687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACT500.007207035628.000864
GAGTGTC906.1760747E-627.2188847
ATCTTTT10650.026.28785312
TATGGGG808.6632135E-526.2508072
AATAATA1207.397284E-826.2508052
GTCATAA959.381882E-625.7922441
TCTTTTT10750.025.06669213
TTTCGGA2001.8189894E-1224.5026321
AAACGTA1451.7273123E-824.13682260
AGACTTA901.926224E-423.33583850
CTTTTTT11700.023.0313614
GATCTTT12600.022.77498411
AACGTAA1703.752575E-922.64601961
GTGTAAA800.002664258521.877351
TATTATG800.002665445221.8756712
TATAGAG800.002665445221.8756715
TCTAGGC800.0026678221.8723168
TGCATCG4500.021.78178670
GTCTTAC4250.021.4140641
GTACTGT1154.117455E-521.3033686
Produced by FastQC (version 0.11.5)