FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605548
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT81400.22576318495829203No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA60270.16715905598816047No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG56690.15722991345559675No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT53910.14951957372360594No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT53110.14730077092303306No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC50900.14117132818645045No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT49670.1377599188805696No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA49220.13651184230524735No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC49040.13601261167511847No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG48850.1354856460099824No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA45030.12489086263724682No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG40520.11238236184901713No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA39190.10869360219306469No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA38990.10813890149292146No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT38550.10691855995260637No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA36580.10145475805619561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC22800.023.94367410
TTTCGGA11150.023.8591081
TATGCTT23200.023.838155
ATATAAG15300.023.3355562
TCTTTTT67050.022.8078148
CTTGTCT24300.022.4656689
TATATGC23300.022.3837973
CTTTTTT70350.021.8871679
AAGATTA25100.020.91455718
AGAAACG29550.020.84743367
AGATTAA25650.020.46552819
CGTAAAT17100.020.46467823
TATAAGA17900.020.3368513
ATCTTTT80400.020.2829387
TGCTTGT26950.020.2566137
ATGCTTG28350.019.8781626
GTCTCAA27700.019.70869612
AACGGGT34400.019.63359622
ACCGGTA17900.019.5514115
ATGTCTA26400.019.49076533
Produced by FastQC (version 0.11.5)