FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949619
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC20440.21524421899730312No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC19090.20102799122595486No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC18080.19039214674516833Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC15830.16669843379292115No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15700.16532946371123577Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC13810.14542674483134815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAC952.824163E-729.4731755
TAGGTTA604.973405E-429.1769248
TTTCGGA1800.029.1646271
TCTTTTT14950.026.9211928
ATCTTTT15200.026.478417
ATAAGGT808.6701344E-526.2495464
CTTTTTT15900.025.3126939
TATTACA851.3089397E-424.70545666
TTCGGAA2350.023.8281212
TTACACC901.928755E-423.332934
CTGATAT750.001833543423.33170128
TAGTCCA750.001834105123.33047317
CGTGTGG21800.023.12008570
GATCTTT18700.023.0209926
GCCTATA952.7802476E-422.10604769
TACGGTG1304.3462314E-621.53695510
TATCTCG2800.021.2507570
GACCGGA35250.020.75250670
TTAGCGG850.00379066620.587885
GATACCC1205.7194284E-520.415247
Produced by FastQC (version 0.11.5)