FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378155
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA1154.9058144E-827.3914322
TATCTCG4900.027.14396970
ACAGTCC1101.0180756E-625.4518958
GTATAAT2650.025.096281
CGTGTGG13150.024.48769670
CATACGT1652.6630005E-923.3334434
TTTCGGA4700.023.086891
TACTATA4250.022.23542
GAGCATA1905.638867E-1022.1069721
AGCATAC1807.2723196E-921.3889882
CTTTTTT22900.021.0895049
CTAGACT1003.9340556E-421.0000974
CTATTAG850.00378944520.5898271
GTACAGT850.003791038420.5883336
TCTTTTT23450.020.445638
TGTCTAA3300.020.15307234
AGAGTCG1801.7053208E-719.4424256
ACTATAC2900.019.3104343
CCTATCT10350.019.27615270
CTATAGT1107.474269E-419.0923841
Produced by FastQC (version 0.11.5)