FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611285
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG5850.023.33403870
ATCTTTT37750.022.0648357
CCTATCT13450.021.85939670
GATCTTT41950.021.7772566
ACTAGAC1859.968062E-920.8120863
TCTTTTT40200.020.633038
CTTTTTT40250.020.3459139
GCCTATC16950.018.37813669
CTAGACT1752.7959632E-618.0011024
GATCTGG37600.017.6872526
GGGGTGC20000.017.5010726
TATAGTA2601.4606485E-917.5010722
TTTCGGA5850.017.351491
AGCCTAT19450.016.91567668
ACGAGTG4400.016.7050569
CTGTGCG2954.3655746E-1016.6086089
CGTGTGG15600.016.378770
CTAGACA4550.016.1548354
CGATCTT79300.016.0665555
TTCGGAA6800.015.9568592
Produced by FastQC (version 0.11.5)