FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050448
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAT552.993664E-431.817493
ATACAGT500.00721085627.9993926
TAATATA808.671259E-526.2494284
TATCTCG4350.025.75024470
TAAGCTA851.3069942E-424.71122732
TTTCGGA3450.023.3339371
ATTATTC1052.0407542E-523.3328253
ATCTTTT21400.022.4039237
TCTTTTT21100.022.3907478
CTTTTTT21350.022.1285619
CGTGTGG11900.021.7673470
GTATACG1304.339763E-621.5410731
AATTAGT1154.115232E-521.30692731
GATCAAT1356.0299462E-620.73831611
GTATAAG1205.7159556E-520.4171921
GCCTATA1558.7376065E-720.3250470
ACATTAG1055.4669305E-419.9995635
AGTCGTA1055.470439E-419.99766217
GATCTTT25550.019.8625816
TAAGGAT1257.826854E-519.5995735
Produced by FastQC (version 0.11.5)