FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630556
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATA1200.037.9194562
TATCTCG4950.026.86901770
GTATAAT1358.085408E-925.9286291
ATCTTTT23800.022.79312
TCTTTTT24000.022.1676213
GTATCAT952.7801358E-422.1075691
AATTAGT1604.911817E-821.87593831
CTTTTTT24350.021.70524414
CTAGTCG9650.021.39791320
CACTAGG1807.2668627E-921.3911191
CCTAGTC9950.020.7546619
ACACCGT11950.020.5023446
TACTAAC8300.020.2430741
GATCCCT10600.019.81217215
ATAATAC1601.186896E-619.687743
GTCCGAT11500.019.4767111
TAGGGTC1451.1175203E-519.3105814
TAGTTTA10850.019.03249230
GATCTTT30950.018.9986176
CTATAAT1852.2709355E-718.920891
Produced by FastQC (version 0.11.5)