FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637080
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT176850.029.9284842
CGATCTG69250.029.6193835
TCCGATC185450.028.6700953
ATCTTTT40850.028.6145067
CCGATCT183750.028.5353134
GATCTTT42050.028.4672186
TCTTTTT43200.026.8959088
CTTCCGA204800.026.0840971
CTTTTTT44350.026.040679
CGATCTT65250.025.6416265
GATCTGA20150.024.8396766
GATCTGG34950.024.5360036
CGATCTA32000.024.5017015
CTAGACA1151.4975703E-624.3495144
GTATAAG1802.8558134E-1023.3378031
ATCTGAC6750.023.3313887
ATCTGAG8900.023.2003147
TATACAG4100.023.050385
ATCTGGG16250.022.8288657
TTTCGGA8300.021.9319111
Produced by FastQC (version 0.11.5)