FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888313
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG24040.12730940262551813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG11550.026.36108220
ATCTTTT43150.023.76363212
TCTTTTT43300.023.68193613
CCTAGTC12950.023.24160819
CTTTTTT44800.022.81089614
TAGTTTA12850.022.33424230
AGTTTAT12900.021.97636231
TACTAAC11850.021.8567391
GTCCGAT13600.021.61555511
ATAGTTT14100.021.59365329
GATCTTT51300.021.5590786
GATCCCT13650.021.53694715
ACACCGT15000.021.4662916
CGGCATA13650.021.2799925
CGTGTGG24300.021.17471570
GAATAAT4350.020.9197311
TCTGAGC5900.020.761258
ACACAGT1901.3564204E-820.2628066
GCATAGT14950.019.8977427
GTATAAG2152.6338967E-919.535061
Produced by FastQC (version 0.11.5)