FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917468
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC55480.28933990032688944No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC48690.2539286183654695No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT41940.21872594483975744No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC39780.2074610893115296No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC37940.19786510126896512No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC32310.16850346394307492Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG29040.1514497243239522No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA28250.14732970771872073Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA27110.14138436730104492No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT26200.13663852538868967No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC24380.12714684156397918No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA24370.12709468945505217No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT23380.12193163067128107No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC22160.11556907338218943No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT21850.11395235800545302No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG20730.10811132180563118No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA20520.10701612751816457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCT7150.025.94452970
TTTCGGA3400.025.7371061
CGATACA700.001226910224.9998044
AACGGGT15350.024.6239322
GGGTAAC15300.024.47565725
GGTAACG15650.023.92890226
CGTGTGG31150.023.8206670
CAGGGTT15350.023.25714939
AGGGTTC15200.023.2557940
CGGGTAA16400.022.83399624
AGTAATC8800.022.67086869
GTAATGT4650.022.58105770
GGTTGCA16500.022.48350116
GTAACGG16750.022.35745227
GGGTTCG16150.022.32064241
TCAGGGT16050.022.02589838
TAACGGG16650.021.8611228
GTTCGAT16500.021.85116443
GGGGAAT16600.021.71839732
GAATCAG16450.021.70422235
Produced by FastQC (version 0.11.5)