FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340321
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACCATTGGGGCAAGGTCATCAGTAGGGTAAAACTAACCTGTCTCA25540.19055136791858068No Hit
GCTCTATCCCCAGCACGACAGAGTTTAACAAGATTACCCAGACCTTTCGG22030.16436361140353692No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAATTTGACGAT18750.13989186172566123No Hit
GCCATAATCCAGCAGATGGTAGCTTCGCGGCAATGCCTGGTCAGACAGCC17480.13041651962477646No Hit
ACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGTAA16980.1266860699787588No Hit
TGGTAATACCGCTTAGTACGAGAGGAACCGCGGTTTCAGATAATTGGTTT16630.12407475522654648No Hit
ATCCAGCAGATGGTAGCTTCGCGGCAATGCCTGGTCAGACAGCCGCAAAA15800.11788220881415722No Hit
GTTTACGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT14820.11057052750796265No Hit
GACTTAGAGGCGTTCAGCCATAATCCAGCAGATGGTAGCTTCGCGGCAAT14730.10989904657167948No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT14710.10974982858583876No Hit
GTTCTGGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCAGAT14450.1078099947699096No Hit
CTACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGT14310.10676546886902466No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACAGAGCCAACGAGTTCTTCACC13430.10019987749203363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA450.0043092131.110382
TAACGTG604.9865025E-429.16489410
TAATGTC1004.423582E-728.00143269
CAATACT755.5910685E-527.9982994
GATCTGG35800.026.2972856
GTACATG1358.092684E-925.9253181
AGGGATG1508.8402885E-1025.6651065
GGGTATT5950.025.29446816
ATCTGGG16050.023.986087
ATCTGTG7050.023.8283397
GATCTTT23150.023.735066
ACATTAG750.001833837523.3319155
CGTGTGG10500.023.00117770
ATCTTTT20350.022.8733027
ATCTGAG6450.022.7893147
CGATCTG105850.021.6897545
CTATAGG2108.367351E-1121.6661591
TCTGTGC5200.021.5371538
CTAGGGG3250.021.5371533
GTCTAGG2451.8189894E-1221.4280681
Produced by FastQC (version 0.11.5)