FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486884
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAATCCCTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGC35680.239964919926504No Hit
GAGTTATCGTTGCGATAACTAGGCGGGATTCTGACTTAGAGGCGTTCAGT31960.21494615585344923No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC25520.17163410192052642No Hit
GTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGCCGAT21210.14264730806169143No Hit
GACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCATT20330.13672889075408706No Hit
GGATTACTATTGCAACAACGAGTCATCAGTAGGGTAAAACTAACCTGTCT20140.1354510506535816No Hit
CTCTCGTACTGAGCAGGATTACTATTGCAACAACGAGTCATCAGTAGGGT18540.12469029191248275No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTGACGAT16570.11144110771250482No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGACTCGTTGTTGCAATAGT15980.10747307792672461No Hit
TCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCACC15830.1064642567947466No Hit
CCATAGCCCCGTCTCGACTGCTTGCAGTGGGGCGGAGGCAGAGCGTACAC15780.10612798308408725No Hit
CCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGCTTCGCAAT15730.10579170937342793No Hit
CCATTGGCCGATCGGCCAAGCACATGAACCAAATGTCTGAACCTGCGGTT15670.1053881809206367No Hit
GAATTATTACTGTTCTGGATTCTTCTTAGAGGGACAAGTGGTTTTCAGCC15380.10343779339881255No Hit
CTTCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCA15360.10330328391454882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTG5200.031.6311827
GTATAAA1001.26092345E-831.499761
CGATCTG67600.030.648595
GTATAAC604.985213E-429.1664411
CCGATCT175500.027.7984874
TTCCGAT178050.027.3619692
TCCGATC179150.027.3102633
GATCTGA19000.026.3401326
GATCTGG31350.026.2340776
ATCTGTA4300.026.0436847
CTTCCGA189050.025.7337341
TCTGTGC5050.025.6407818
CGATCTA32400.025.5999345
ATCTAGG4900.024.9972867
ATCTGAC7750.024.8360127
GATCTTT28800.024.789816
ATCTTTT27450.024.6057077
CGATCTT47150.024.4202275
TATAGTA1151.49886E-624.3468192
GATCTAG10750.024.0912196
Produced by FastQC (version 0.11.5)