FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063047
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT30250.024.6467326
TCTTTTT27750.024.4671048
ATTATTC4600.024.3499033
ATCTTTT28400.024.1535827
TATGACT1902.1827873E-1123.9494134
TTATTCC5100.023.3353254
CTTTTTT29100.023.2117639
ATTCCAT5000.023.101976
CATACGT1253.0881147E-622.4029642
CGTGTGG13000.022.34701270
TAATGTA5200.022.21029915
TCGTATG2056.002665E-1122.19388440
TATTCGA4900.022.14162320
AGTAATG3800.022.1061170
ACTCTAT800.002665694821.87686750
AGCATAG5000.021.7008326
ATTCGAG5050.021.4839521
TTTCGGA3600.021.3917221
TAGGACA1154.1164265E-521.3061664
GCTAATG5550.020.8096513
Produced by FastQC (version 0.11.5)