FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315069
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTA200.006944325852.50583329
GTACCAT200.006948686752.4974986
GTATTGT352.0845811E-549.9976231
ACGAATT350.001253696440.00444817
ACACATA501.7081498E-434.99833370
GGTAGTG450.00430476531.1096359
TTAATCT500.00720422527.99866760
GGCACGT500.00720422527.99866724
TGGACCG959.37308E-625.7882445
ATCTTTT12600.025.5543387
TGATCTA700.001224918524.99881220
TCTTTTT12950.024.8636828
AGTAATG1851.4551915E-1124.59342470
GATCTTT13400.024.551076
TCAGGGG1001.3950903E-524.4988333
ACTGTGC901.9251731E-423.3322248
CTTTTTT13650.023.3322229
ATGCTCT1502.4652763E-823.3322221
GTACTGG1052.0352922E-523.3322221
AGTAATA750.001830599623.33222270
Produced by FastQC (version 0.11.5)