FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727705
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC20270.11732326988693094No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC19590.11338741278169595Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA19450.11257708926002993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGG22350.027.40845770
GTAATGT2700.025.92927270
ATCTTTT47300.025.008737
TTAGACT700.001226916824.9996094
CTTTTTT48500.024.0291619
GATCTTT53100.023.7284416
TCTTTTT49200.023.687288
ATAAGAC1403.289424E-722.4996473
TAATCTC5750.021.91523769
GTAATCT9900.021.5684470
GCCTAAT1304.3465043E-621.5387461
TTTCGGA6400.021.3284071
CCTATAG850.0037918720.5879123
AGTAATC10650.020.37762669
CATACGT2252.1282176E-1020.2219034
AGTAATG5900.020.17209470
CAGGGGT5950.018.8232354
TTCGGAA8350.018.0241892
TGGCGTA5050.018.02212750
TAATGTC1752.7951628E-618.00180269
Produced by FastQC (version 0.11.5)