FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC21.5384615384615385No Hit
GCACCCCCTTTGAGATCGGAAGAGCACACGTCTCCCATGGCAGATCGGAA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGAAATGAGACAACTTGCAAAAGATCTGAACCTACAGACTATTCCTCAG10.7692307692307693No Hit
ACGCAAACGAGTGCACTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
CGTCTCCCTTGCGAGATCCGAAGAGCACACGTCCTCTCTCTAATACACAT10.7692307692307693No Hit
ATGCTGACCTTCATTAAAGAGCTAAGAGGGCAATACCGCTTACATGAAGC10.7692307692307693No Hit
TAGTACGGCTCGGCTAAACACATTGCTGTGCGTACACCTACCGCCTATCA10.7692307692307693No Hit
GAGCACACGTCTCCCAAGCGAGATCGGAAGAGCACACGTCTCCCAAGCGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTCCGGGAGACGTGTGCTCTTCCGATCTCCTCCGGGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATAGGGAGACGTGTGCTCTTCCGATCTCAATAGGGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTCCCAACGCAGATCGGAAGAGCTGTCTCTTATACACATCTCCGAGCC10.7692307692307693No Hit
GTGCTCTTCCGATCTGGCGCGGGGTGCTCTTACGAAAAAAAAAAAAAAAA10.7692307692307693No Hit
CCGATCTACCTAGGGAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC10.7692307692307693No Hit
CTATTAGAAGAAAACATTGGAGAAACAATTCAGGGTATTGGTCTGGGTTT10.7692307692307693No Hit
CTAACGCGGGGTCTTAAAGTCCTAAAAAAAAAAAAAAATAAAAAAAAAAA10.7692307692307693No Hit
TTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGTGATTAAATAC10.7692307692307693No Hit
ATGTCGAGGTCAAGGTGGTGGATGCCGAAGGCGGCACGGTGCCGGTCGGC10.7692307692307693No Hit
TCCCTGTGTAGATCGGAAGAGCACACGTCTCCCTGTGTAGATCGGAAGAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCATTATGTTAAGACAGGCAGAGAAAGACAAACTTCACATGTTCTCACTG10.7692307692307693No Hit
CAACTGGCAAGCCCAAGGGAAAGTAAGATCGCAAGAGCGTATTCTACCGA10.7692307692307693No Hit
ATTTAGGCTTATCGGGTGGTCCCGACAGATTCACACCGGATTTCACGGGC10.7692307692307693No Hit
GAAGAGCACACGTCTCCCATTGCAGATCGGAAGAGCACACGTCTCCCAGG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCCAGCCAGATCGGAAGAGCACACGTCTCCCTTCCAAGATCCGAACAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AATCTCAAACGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GACGAATAAACTACAGATATCAACAGGATACCTTTTATCATAAGATCTCA10.7692307692307693No Hit
CTCGCCGCCGCGGCGCTGCCCGCGCGACTCGCCACCCTGGGGCGCCCGAT10.7692307692307693No Hit
TACTACCACTCAGCCTCCTGAGTGCCACGGACTGCAGATGTACTACACCA10.7692307692307693No Hit
CTACTCTCCTAACTGATACTACTTCCTCATTTCAATCCTGTCTCTTATAC10.7692307692307693No Hit
TAATAGCTCACTGGTCAAGTGATTCCGCGCCGATAATGTAGCGGGGCTTA10.7692307692307693No Hit
CTACGCCACCGTGCTGAACGGCCGCACGCCGCTGGACCACGGCATCCTCG10.7692307692307693No Hit
GCACACGTCTCCCACACTAGATCGGAAGAGCACACGTCTCCGGGACAGAT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCAAACCAGAGCACTTCCTTAATAAACTACGAGACGACATCTCTAACGAA10.7692307692307693No Hit
CTCCGTGGGGGATTGGAAGAGCCCACGAGACCGATCAGTATCGGGGTTGG10.7692307692307693No Hit
GCTTCCATCGCTTTTACCAATTGCGAGTTAGTAGAAGCGGAATTTTCGCA10.7692307692307693No Hit
ATTTTCGCCTGATCGCGACGGCCCAGGAACAATTCCGCCAGAATCAGCAG10.7692307692307693No Hit
ACGCGTGTGCTCTTCCGATCTTCCTTGGGAGACGTGTGCTCTTCCGATCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTTCCGATCTCCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGGGAGACGTGTGCTCTTCCGATCTGTGTTGGGAGACGTGTGCTCTTC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACGTGTGCTCTTCCGATCTAGCTAGGGAGACGTGTGCTCTTCCGATAGA10.7692307692307693No Hit
CCATTGGGAGACGTGTGCTCTTCCGATCTCCATTGGGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGCACACGTCTCCCGGAGGAGATCGGAAGAGCACACGTCTCCCGGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATATAACACAATCTTGACTTTGACAGGCTCGCTGCCCATGCGCTTGAT10.7692307692307693No Hit
TCCCGGGTCAGATCGGAAGAGCACACGTCTCCCTCAGCAGATCGGAAGAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATTGAGCGGCTGCGGGCTGGCGGACAGCCACGCGGTGTGGCCGGAGATGT10.7692307692307693No Hit
GTTCAAATACAAAAAATTTAAAAATTATAAAAAATTTAATTGGAAAAAAA10.7692307692307693No Hit
CTCGATGACCGACACCGCCTGGTTCCTGGAGATCGCGTCTTACGCCTTCG10.7692307692307693No Hit
GGCTATCGCCTTAAACTTGGCACAGTCAGTAAGTCGCAGGATCATGCTTC10.7692307692307693No Hit
AATTAAGGCATAGAGTCTTTATCAGTTTTCTTGTGGTTTATGTATTTGTT10.7692307692307693No Hit
CTTCCGATCTGATCCGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
CCCCTAACACTTGCAATCCCTTGTAAGATCGGTGCTCTTCCGCTCTTACA10.7692307692307693No Hit
AGTAGTGATAGCTTTATGGAATTGTTTATTTTTAGTTTGGCAAAGGCTAA10.7692307692307693No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGAAAGGGGCCCGC10.7692307692307693No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCAGCGCGGTTGCCGGCGT10.7692307692307693No Hit
CGTCTCCCAGTGCAGATCGGAAGAGCACACGTCTCCCAGTGCAGATCGGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAAAAAGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GTATACGGACTGACTCCTGCCCGGTGCTGGAAGGTTAAGGGGAACTGTGA10.7692307692307693No Hit
ACAAAGTACTTCTCATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GATCGGAAGAGCACACGTCTCCCAAAGCAGATCGGAAGAGCCCACGAGAC10.7692307692307693No Hit
CACTTGGGAGACGTGTGCTCTTCCGATCTCACTTGGGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGCGCT10.7692307692307693No Hit
ACCCCAAGGATCGAGGCATAGATGCCGAGCAGCCAGCCCAGTGCGAGGAA10.7692307692307693No Hit
CTTCCGATCTCATTAGGGAGACGTGTGCTCTTCCGATCTCATTAGGGAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCTCACTTGGGAGACGTGTGCTCTTCCGATCTCACTTGGGAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAGAAAAAAAAAAAAAAAAAATCGAAAGACCCCCAGGAAACATCAGA10.7692307692307693No Hit
CCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT10.7692307692307693No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGATATTTGAATGATGCGTCAC10.7692307692307693No Hit
CCCCAATCAGATCGGAAGAGCACACGTCTCCCTAGGCAGATCGCAAGAGC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATATACCAAAAACCCAACACTAATAACAAAAACACCAAGGCAATAACTT10.7692307692307693No Hit
CCACAGGGAATGAGACCTCATTCAGAAAAATAAGATCTGACGAAAAGGTT10.7692307692307693No Hit
TCGCAAAGCGATGGGCCTCGTCGCGGATCTGCGCGATCAGCATCAGCGCC10.7692307692307693No Hit
CCCCCGAGAGATCGGAAGAGCCCACGAGACATGCGCAGATCTCGTATGCC10.7692307692307693No Hit
GATCTTGTCTGGGAGACGTGTGCTCTTCCGATCTTTTCTGGGAGACGTGC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACCCCGGGAGACGTGTGCTCTTCCGATCTACCCCGGGAGACGTGTGCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GAGCACACGTCTCCCTGAAGAGATCGGAAGAGCACACGTCTCCCTCTGTC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTATGGCCC10.7692307692307693No Hit
CAGTTACTTATTAGCTATGAAACCCTCCTGAGTACCTTGGTTTTTTCCTG10.7692307692307693No Hit
CACGTCTCCTGGGTAGATCGGAAGAGCCCACGAGACGCGTAGTAATGTCG10.7692307692307693No Hit
CGTCTCACCCTGGTAGATCGGAAGAGCACACGTCTCCCCCTGGTAGATCG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAAGGAAGCGCAGCGAGGAGGTGCGGCCCCGTACCCCCTGGTGCGGG10.7692307692307693No Hit
GTGCTCTTCCGATCTAAGACGGGAGACGTGTGCTCTTCCGATCTGTGCAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACATCGAACCGCTGCAGTTGTCGTTTTCAGCACATAAATCAAAAGGAGTC10.7692307692307693No Hit
GGAATAGTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGTTCTCCAAG10.7692307692307693No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC10.7692307692307693No Hit
TTGCTGGCTAGGACGGCGGCAGACATTCGCCAGCGTTTCGGCGTCGACGT10.7692307692307693No Hit
TAACAAGGTGCTATTTTAAAGCAATATGATCAGGATTCGTAAGCTTTATA10.7692307692307693No Hit
CTCCCTAAGCAGATCGGAAGAGCACACGTCTCCCTAAGCAGATCGGAAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCAGATTAGATCGGAAGAGCACACGTCTCCCAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG10.7692307692307693No Hit
CGTCTCCCCTGTGAGATCGGAAGAGCACACGTCTCCCCTGTGAGATCGGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTAGGAAGCCCTCGATCGAAGCTTGGTATGTAGGATACGAACTCAAAAAC10.7692307692307693No Hit
CTCCACGACCCTGACCGATTCCGCCAGATCGGCTACGCCGAAGCTCACGG10.7692307692307693No Hit
GAAGAGCACACGTCTCCCCATGCAGATCGGAAGAGCACACGTCTCCCCGT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTCGTACGGGAGACGTGTGCTCTTCCGATCTCGTACGGGAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTATTGACCGCCCGCACCTTCTACGTAGCTCCTTCGAAGAGTCGAGTTG10.7692307692307693No Hit
CCACTGGACAGATCGGAAGAGCACACGTCTCCCGCCTGAGATCGGACTGT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCATATAAATACACGCACGATCAACCAAAAACACTAAAAATATCGTAAT10.7692307692307693No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAATCTTACAGTCTGTCACAATTAAA10.7692307692307693No Hit
CCTTTGTCGCGCTTGAAAAGGACACGGGAGCGCTCGCCGGAATTGGCCGG10.7692307692307693No Hit
GTCCCGCACCCGAAACTCGGCGAGAACGTTGCCGCCGTCGTGGTGCTGCG10.7692307692307693No Hit
AAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
CCCATATGAGATCGGAAGAGCCCACGAGACGCGTAGTAATCGCGTATGCC10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCACGAGACATGAGCAGATATCGTATGCCGT10.7692307692307693No Hit
CACAAGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
TCACAGGGAGACGTGTGCTCTTCCGATCTTCACAGGGAGACGCTGTCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCTCTCAGGGGTTTTTATAGGCACAGGATAGGGGCATGGCAAGCCAGGGT10.7692307692307693No Hit
GTCTGGACCTGGTAAGTTTCCCCGTGTTGGGTCAAATTAAGCCGCAGGCT10.7692307692307693No Hit
ATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCAAGGCTTGACATG10.7692307692307693No Hit
GACGTGTGCTCTTCCGATCTGTCGAGGGAGACGTGTGCTCTTCCGATCTG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTTATACGTCGTATAATTGGAATGAGTACAATTTAAATCCCTTAACGA10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10.7692307692307693No Hit
GATCTGAGCTGGGAGACGTGTGCTCTTCCGATCTGAGCTGGGAGACGTGT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCTAGCCTGGGAGACGTGTGCTCTTCCGATCTAGCCTGGGAGACGTGT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGTTTCCACAGACGGCGTGCTGTGGATACCCTCTATTCGCAGCGACGAA10.7692307692307693No Hit
AACCAAGAGCGCACCTTTCGCGCGTACTGGTTCGAGACTGGCACCCCCAC10.7692307692307693No Hit
TCCCAAGACAGATCGGAAGAGCACACGTCTCCCAAGACAGATCGGAAGAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCTCTGTCTCTTATACACATCTCCGAGCCCACGA10.7692307692307693No Hit
GACGTGTGCTCTTCCGATCTGGACTGGGAGACGTGTGCTCTTCCGATCTG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACCCCGTCGAGATCGGAAGAGCACACGTCTCCAAGCGATAACGGAAGAGC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATAAACACATATACGAGCCCACGAGACATGCGCAACGCACCTATACAGT10.7692307692307693No Hit
CACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGCTGTCTCTT10.7692307692307693No Hit
AACCTCTCCAAAACAAAATAGCAAAAGAAAACCTCTCCAAAACAAAATAG10.7692307692307693No Hit
GTGCTCTTCCGATCTGGATTGGGAGACGTGTGCTCTTCCGATCTCGATTG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGCCCACGAGACCGATCAGTATCTCGTATTGCGTGTTGTGCTTGATAAA10.7692307692307693No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGAGCCC10.7692307692307693No Hit
CCATAGGGAGACGTGTGCTCTTCCGATCTCCATAGGGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACTGCAGTGATTGTTATTGCTCTTCTGGGTCTAGCCACCCACAGGGGC10.7692307692307693No Hit
CTTCCGATCTGAGACGTGTGCTCTTCCGATCTGAGACGTGTGCTCTTCCG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTCACATGGGCCGATCTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA10.7692307692307693No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers