FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237010
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA200.006954862352.49484610
CGAGTAA700.001227020424.99855859
CGTGTGG5950.023.53185870
TACACCG901.9297434E-423.3319855
TGGACGG1253.0949814E-622.39780210
ACGGTCC1356.0303955E-620.7387059
GTACTGC1709.3317794E-820.5862146
GATCTGG17600.020.2820996
TACGACG1901.3560566E-820.261995
ATCTGGG9050.020.1085227
CGTCGTA2101.9626896E-920.00207733
CATCTCG2607.2759576E-1118.84811270
TCGCGTC1301.05687635E-418.84811230
TGTCGCC1301.05851665E-418.84430310
TTACGAC2053.2443495E-818.7794024
CGATCTT21650.018.4284975
CGATCTG53500.018.3821165
ACGACGG1351.4136152E-418.1463666
GCCGGGA1551.9850977E-518.06420313
GTCGTAA2207.265044E-817.5018234
Produced by FastQC (version 0.11.5)