FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168277
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT69230.3192857739117281No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA51250.23636278944064804No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG49860.22995216939533095No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC46960.21657749448064065No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT46470.21431763561574466No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT45180.20836821125714106No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT44380.20467864576343336No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA43270.19955937364091397No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC42390.19550085159783553No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG40890.1885829162971336No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA38790.17889780687615098No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT37610.17345569777293215No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG36220.16704507772761507No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA35210.1623870012918091No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA33550.1547311528923657No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT31690.14615291311949533No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA31490.14523052174606843No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA31290.1443081303726415No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA30040.13854318428872325No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC29110.13425406440228807No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA28490.13139465114466464No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG27730.12788956392564232No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA27530.12696717255221543No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC25640.11825057407333103No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC25520.11769713924927487No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA25360.11695922615053336No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC25150.1159907152084351No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA24870.1146993672856374No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT24380.11243950842074145No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA24250.11183995402801394No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA23980.11059472567388762No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA22300.10284663813710149No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGT22290.10280051856843014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG657.9622364E-426.9227223
TACATCT13800.022.5758170
GGGTAAC25650.022.51276625
GGTAACG25850.022.47449126
TAGGACT27500.022.3997044
GAATCAG26300.022.35918235
GACTACC27650.021.7703577
AACGGGT26550.021.7496222
AGGACTA27700.021.7325655
TAACGGG26550.021.61830328
AATCAGG27000.021.3905836
CAGGGTT27000.021.2599639
TGCAACG27200.021.2303619
GTAACGG27450.021.164527
GCAACGG27750.021.06181520
GGAATCA27600.021.0523934
TCAGGGT27300.021.0268238
ATATAAG15000.021.0002082
AGGGTTC27100.020.92319740
AGGGGTG8900.020.842425
Produced by FastQC (version 0.11.5)