FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768351
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT39900.024.8224077
GTCATAT1851.4551915E-1124.596631
TCTTTTT40550.024.2519028
CTACTAG2200.023.8656141
CTTTTTT42100.023.3583569
GATCTTT46600.022.8358026
CGTGTGG24700.022.67394370
TTTCGGA5400.022.0388621
TAATCTA800.002665989321.877435
AGTCACA850.003794089420.58586553
GATTAGT2101.9699655E-919.99713315
GAGCATA2555.4569682E-1119.2172781
TATTAGT1651.5924488E-619.0930294
AGCATAC2053.2405296E-818.7825742
TACATCT14600.018.70017870
TAGGGGT6750.018.6687414
ATTCTAA2508.6583896E-1018.2015081
TCTAGGG7350.018.0972482
TATAGGA1752.7949445E-618.0022
CTAGACT1953.94808E-717.9507124
Produced by FastQC (version 0.11.5)