FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727556
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC28270.16364158383288296No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC23200.13429376529617562No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC21660.12537943777220537Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA20790.12034342157359876Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC18750.10853483186652126No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC18640.10789809418623768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6300.026.6697771
TACATCT13750.024.43779870
ATCTTTT52300.023.9551837
GATCTTT57450.022.9684816
CTTTTTT54700.022.968119
TCTTTTT54150.022.9428848
TTCGGAA8050.022.1758562
ACATTAC1003.9339447E-421.0006263
AATAGTC1003.9377905E-420.9975857
TAAGAGT1709.3245035E-820.588854
ATGCTCT7050.020.3569851
GTATAAT1751.2648707E-720.0023331
CGTGTGG20350.019.6080370
TATAATG2104.2715328E-818.3349422
CTACATC19300.018.1357869
CAGGGGT7750.018.0650544
CGATCTT111000.017.9419775
TCTAGGG5750.017.653722
AATCTAG1401.8646987E-417.502041
TAGACAG4800.017.500525
Produced by FastQC (version 0.11.5)