FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397796
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG18830.13471207529567977No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT18450.13199350978254337No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA15010.1073833377688876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA4650.024.08542815
ATATAAG4750.023.58092
GTTATAC901.9280807E-423.3352663
GATCTGG28950.023.2143576
CGGGAAC5450.022.47651115
TATAATA1253.0905994E-622.4018552
TACATCT4700.022.34067570
GTCCTAG5400.022.0388621
GTATTAA1454.606427E-721.7259371
CTAGGAC7750.021.6792133
ATCTGGG13250.021.3957027
TCTAGGA8200.021.343232
TCCTAGA6100.021.2312662
TTGTCTC6600.021.21008510
AGGACTA7600.021.1859665
AACGTAT5950.021.17595119
CATATAA5300.021.1338251
TATAAGA5300.021.1338253
CGATCTG83000.021.1282565
GATTAGG1506.3823063E-721.001741
Produced by FastQC (version 0.11.5)