FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n01_hr38s10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n01_hr38s10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGACTGACTCCTGCCCGGTGCTGCAAGGTTAAGGGGAACTGTGA10.6993006993006993No Hit
CTTCCGATCTAGCCGGGGCTCTTCCGATCTTTTTTTTCAAAAAAAAAAAA10.6993006993006993No Hit
ATTCCGATCATTTATATTATATTTTTTTTTTTTTTATTAATATTAATTTC10.6993006993006993No Hit
TACCAAAAGTGGCCCACTGGGCACATTATATCATAACCTTGAACTTCATA10.6993006993006993No Hit
GTTCAAGGCGGCGCTGGTGTGGGACGCCGAGGCGGGGAAGGTGAACTTCG10.6993006993006993No Hit
GCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGAT10.6993006993006993No Hit
GCTGGGGATAGAGCATTGCAATTATTGCTCTTCAACGATGAATGCCTAGT10.6993006993006993No Hit
AAATACATCATCAAGGAGAAGCCCGACGCGAAGATCGGCGTGCTCTATCA10.6993006993006993No Hit
CAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGGTGGGCGCTAGGTG10.6993006993006993No Hit
CCACTATGCCGATCGCAAGGCACTGCCGCTCGACCGCGTCACGGTCACGC10.6993006993006993No Hit
AAAAACCATTAAAAACCATTAGAATCCCAAACATTACATACAAATACATA10.6993006993006993No Hit
GATCGGAAGAGCACACGTCTCCCAGGTCAGATCGGAAGAGCAAACGTCTC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTTTATTTTTTTTTTTTTTTTTTTTTTTTTATTTAGTAGATGAAGAAAA10.6993006993006993No Hit
ATAATGGGAGACGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
CTTCCGATCTTCCATGGGATACTATTCCTAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
TCCCAGAATAGATCGGAAGAGCACACGTCTCCCAGAATAGATCGGAAGAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAACA10.6993006993006993No Hit
GTATCAACGCAGAGTACATGGGAGATCGGAAGCTGTCTCTTATACACATC10.6993006993006993No Hit
CTTCCGATCTGGTCAGGGAGACGTGTGCTCTTCCGATCTACACGGGGACT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAATCCGCTAGACTATTAAT10.6993006993006993No Hit
AAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTT10.6993006993006993No Hit
CGTTGAGACAGTGCCCAGATCGTTGCGCCTTTCGTGCGGGTCGGAACTTA10.6993006993006993No Hit
CGCCCCAGCAGCTTGCCGTCCCGGCTCGTCATCGCGAAGGGCGGCACGGT10.6993006993006993No Hit
ATATAAAGTCAAACCTGCCCACTGAAAATTTTAAATGGCCGCACTATTTT10.6993006993006993No Hit
CACAGGGAGACGTGAGCTCTTCCGATCTGCACAGGGAGACGTGTGCTCTT10.6993006993006993No Hit
CTTCCGATCTGGGCCGGGGTGTGTGCTCTTCCGATCCTGTCTCTTATACA10.6993006993006993No Hit
GAGCACACGTCTCCCTGGCAAGATCGGAAGAGCAAACGTCTCCCTGGCAA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACCGGGCCGATCCAGTAACGCAAGGTTCCGTCGGCTGACGGAGATGGCA10.6993006993006993No Hit
GTGATCGGCGTGCCGTTCCAGCACGATCAGCCCGGCGAGCGCGCCTGAGA10.6993006993006993No Hit
GCAAACATCCCAAAGAAGTTTCTGAGAATACTTCTGTCTAGATTTTCTCT10.6993006993006993No Hit
CCCCTCCCGGGGTACTTTTCACCCTTCCCTCACGGTACTCATCCACTATC10.6993006993006993No Hit
CTCGTGGGGGAAGGCCTGGGCCCCGAGATGGCCCTCCTCACCGACGGCCG10.6993006993006993No Hit
GGTCGATGTCGCGGCGAACCTCCATCGCGTCCTTCCAACGCGGATAGAGC10.6993006993006993No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATGTCGTTTGGGGGGGTGTGGGTG10.6993006993006993No Hit
GTCCCGACTAACCCTGATCCGATTAGCGTTGATCAGGAAACCTTAGTCTA10.6993006993006993No Hit
GTTACGAAACGTGAGAACATCACATAAACCGAATAAATACCTAAACTATA10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTAAAACCA10.6993006993006993No Hit
GTTACGTTGTGGGCGAAACAAGCTGAATGGCCGGATCAAGTCCGGCCATG10.6993006993006993No Hit
CCTGAACTCATCCTAAATTCCCAAGCAGCCCAAACAACTTCACATCATCC10.6993006993006993No Hit
GAGATCGGAAGAGCACACGTCTCCCTCGTGAGATCGGAAGAGCACACGTC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCGTCTGAGATCGGAAGAGCACACGTCTCCCTGGGGAGATCGGAAGAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTC10.6993006993006993No Hit
CGTGTGCTCTTCCGATCTTTGCAGGGAGACGTGTGCTCTTCCGATCTTTG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGTTGCTCAGGAGAATGTCGTAGGGTGGGTTAGCCTCACGGATGGCG10.6993006993006993No Hit
GATCTAACCTGGGAGACGTGTGCTCTTCCGATCTAACCTGGGAGACGTGT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCTCTTCCGATCTCAACAGGGAGACGTGTGCTCTTCCGATCTCAACAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAATGAAGACTTTACAAAACTATTGTAAATTGACTCCTCTGAAAATAGA10.6993006993006993No Hit
GCGCGACTCCTGGGGCGCCAACAAGGCCGGCGGCCCCGCCGCGATGCCCA10.6993006993006993No Hit
CTCCCTCGCCGTTTGGGAGAGGGAGCGCGCAGATCTGCCGGTGATGTCAG10.6993006993006993No Hit
ATCGCGATCCGCGAATAGGTCACGATCGCCTTCTGGCCGGCGATCTGGCT10.6993006993006993No Hit
CGTCTCCCCAACTAGATCTGAAGAGCACACGTCTCCTTGGTAGATCGGAA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACGCGGCGGCGATGGCGGCATGGCCCACGGCAACGCTGATCTGGTCCAGC10.6993006993006993No Hit
ATATATAGACAATATAGATCATCCCAACAGCCAACTGATCCTAAAACGAA10.6993006993006993No Hit
CTTCCGATCTCCAAATGGTAGCATTTATCACTTCAGAGATGGCAATTCCA10.6993006993006993No Hit
GAACCGCTGCGAGCCTCCACCAGAGTTTCATCTGGCTTCACCCTATTCAG10.6993006993006993No Hit
CTTCCGATCTCAACCAGGAGACGTGTGCTCTTCCGATCTCAGACGTGTGC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGTGCTCTTCCGATCTGGCCTGGGAGACGTGTGCTCTTCCGATCTCGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCTCAAATAGAGCAGCCACAATCAAACCCTTTTCAAATCCTTCGAGGGG10.6993006993006993No Hit
AAGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATCTTTTCATTAAT10.6993006993006993No Hit
CTTCCGATCTAAGCTGGGACACTACTGAACAAACCCAGAGCACTTATATA10.6993006993006993No Hit
GGCAATGGCTCCATCGGCATCGGCCAGGCGTCCAGCCGGAATACGGCCCG10.6993006993006993No Hit
GTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGA10.6993006993006993No Hit
CACGTGTGCTTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCG10.6993006993006993No Hit
GACGAGTTCCTTGGCCGTGATTCCCTCGAGCGCCTCAGGATTCTCTCCTT10.6993006993006993No Hit
ATCTCGTACAGAGTCTGAAACGCTTTTCTCAACTTGTAGAACCCACGCAG10.6993006993006993No Hit
TAACTTGCTGGTCTGATGTCTCTTGTTCGGTGGTTTCCTCAACAGCTACT10.6993006993006993No Hit
GCTCAGAACTGGTACGCCAAGGGGAATCCGACTGTTTAATTAAAACAAAG10.6993006993006993No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATGTCGGAAACCGG10.6993006993006993No Hit
CAAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT10.6993006993006993No Hit
AGCAACGAAGGGAGAGGTTGCTCCGATTTATATGGTTATGGGAATGGTGG10.6993006993006993No Hit
TAATAAGCCTCATGAACTTGAGCATTTAATTAATGCTGCAACCCTTATAA10.6993006993006993No Hit
TGCCAGGACTTGACGTGGTGCTTGCGCGCCATAGCTGGGGCGGTGATCAG10.6993006993006993No Hit
AAAGTGTACTAATTCCAATTACAGGGCATCGGATATGAGTCCTGTATTGT10.6993006993006993No Hit
ATCCGGAAGCTGCCGCCGTCTCGGCCGCGCCGGCCAAGTTTCGCAACGCC10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTAGCACAC10.6993006993006993No Hit
ATATTGGGAGACGTGTGCTCTTCCGATCTATATTGGGAGACGTGTGCTCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCTGGGCAGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
GGCACGGGTCAGATAGGAAGAGCACACGTCTCCTCTGTCTCTTATACACA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCCAGACGAGATCGGAAGAGCACACGTCTCCCAGACGAGATCGGAAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTGTTGACATCCTGCCATTGGACTTTCGTCCTTGTCCTTGCCAATAGAC10.6993006993006993No Hit
CTCCTTAGTATTCTCTACCTGACCACCTGTGTCGGTTTAGGGTACGGGCC10.6993006993006993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
CGTCTCCCTGGGTAGATCGGAAGAGCACACGTCTCCCTGGGTAGATCGGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGGTAGGACCCAGGGTTCTGTGCAAACACGCCAGGGTAGTCATGGCTCGG10.6993006993006993No Hit
CCCACGGGAGACGTGTGCTCTTCCGATCTCCCACGGGAGACGTGTGCTCT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTATAGGGGAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTT10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTAATTTTAATAAT10.6993006993006993No Hit
TCCAAAGATTTTTAAATTAATAAAAAAAAAAAAAAATAAAAAAGAAATAG10.6993006993006993No Hit
GTATTTAGGCTTATCAGGTGGTCCTGACGGATTCACACGGGATTTCTCGG10.6993006993006993No Hit
ATGCTCTTCCGATCTCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
AACCGCTGAGATTAAGCATATCAATAAGCGGACGAAAAGAAAATTACAAT10.6993006993006993No Hit
GCGTGTGACGGGTGGGAATCCCCTCCGCCGAATATCCAAGGGTTACAGGG10.6993006993006993No Hit
ACCTTAGGATGTGTATAGTTAACACCTCCCGATACGGTGTGATAAATACA10.6993006993006993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTGGTATGGGACACCCCTTG10.6993006993006993No Hit
ATACCCATCTAACACGTCTAAGATCGGAACATTGAAAAAAAAAAAAAAAA10.6993006993006993No Hit
GACGTGTGCTCTTCCGATCTCGTCCGGGAGACGTGTGCTCTTCCTATCTC10.6993006993006993No Hit
CTTCCGATCTACCGGGGGACTGCATGGCCTCGTGCTGGCGGTGCTTCATT10.6993006993006993No Hit
GTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTT10.6993006993006993No Hit
GAACAGCACACCTCTCCCACTAAACATCTGAAGACCACACGAAACCCACA10.6993006993006993No Hit
GAGCACACGTCTCCCCTGGCAGATCGGAAGAGCACACGTCTCCCACAGCA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAAAAATTCTACCTCGATAACTAAACAGCCAAAGCCTATCTAATGAACAC10.6993006993006993No Hit
GAGCACCCCTTGTGAGATCGGAAGAGCCCACGAGCCCGATCAGTATAGGG10.6993006993006993No Hit
AGCCGGAGGCTTGGAAGAGCGTGGTTAGGGCTGAGGTCTTATTGGAGATT10.6993006993006993No Hit
GGCTTCTACGATGTGTCCTTCAAGGGCCAGCCGTTCAAGTTCCAGCGCAA10.6993006993006993No Hit
TCCTGAAAGCGTTCCAAATGTCCACTTCCAGGGAATGCAGAAAGAGTGTT10.6993006993006993No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC10.6993006993006993No Hit
CTTCAGATCTCTGCCGGGAGACGTGTGCTCTTCCGATCTCTGCCGGGAGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCTTCGGGAGACGTGTGCTCTTCCGATCTGACTAGGGAGACGTGTGCTC10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGT10.6993006993006993No Hit
CTTCCGATCTAGGCGGGGAGACTTCTCCACATACCATCTCGCACCGCGTC10.6993006993006993No Hit
ATATTAAGTAGGGCGGAACACGAGAAATTCTGTCTGAAGATGGGGGGACC10.6993006993006993No Hit
CTTCCGATCTCCTTCGGGTTTCAGCTTTGCAACCATACTTCCCCCGGAGC10.6993006993006993No Hit
GGATTGGCCAGTGAGAAATGTTGCGTGAAGGTGAAGCGTCTGGGAAGGCG10.6993006993006993No Hit
GCAGGGCCTGTGCCCGCGTGACCGGCCAGGCAAAGCTGTCCAGGCCGCCG10.6993006993006993No Hit
ACTAAATACTAATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6993006993006993No Hit
TATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGT10.6993006993006993No Hit
ATGCAGGGGTCGGAGTTGATGACGATCTCGTAGGCGAGGCCCATCTGGCC10.6993006993006993No Hit
GGAGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATG10.6993006993006993No Hit
CGCGTAGGTATCGAGTAAATATCGCTCAGATCGCCGTACCGGTAAAGATG10.6993006993006993No Hit
GATTAGAGGCATCGGGGGCGCAACGCCCTCGCCCTATTCTCAAACTTTAA10.6993006993006993No Hit
GTTTAGAGCAGCAGAAACCGGATTTCTGGATAAAAATCCGCCGGATGCTG10.6993006993006993No Hit
CCACGAAGCCGCCTGGAACTACTTCAGATCGTCTGATACCCCGGTCGCCC10.6993006993006993No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA10.6993006993006993No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10.6993006993006993No Hit
CTACAATAACAACAAAAACAAACAACTTAACTAAAAATTGACAGGACTTG10.6993006993006993No Hit
ACGAGAGTTCGCTCGCTCACCTTAGAATTCTCATCTTGACTACCTGTGTC10.6993006993006993No Hit
GAGCAGGGGTGCTCTCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.6993006993006993No Hit
GTTGGAATGGAATCGGTTGTTGTTAAAAAAGATAGCATCAAGGTCCTTGT10.6993006993006993No Hit
GAAAAACCAATGAAAGCATAACAGACATATTATATGTTTTTCCGATTCTC10.6993006993006993No Hit
CCCCACACCAGTGCCACGCTTACAGTTACAGGACTCTCACCCACTCCGGT10.6993006993006993No Hit
GAACAGGGAATGCTTATGCACTGTTGATGAGGTTGTAAGTCAGTTCAGCC10.6993006993006993No Hit
GCACAACACAGCCGGCCCAGCCGCCCGAAAGCTCGGCCACCCAGCGCGTG10.6993006993006993No Hit
GTTTATGTCGTCGCGATCGGCTACGTGGCCCAGAACTACGGCCACGCCTA10.6993006993006993No Hit
CTTCCGATCTCAGCGGGGATTGTCGGCTTTATGGTGGCGCGCATGGGCTG10.6993006993006993No Hit
CCCTTGAACTAACATTCCAATTCGGGTTTTGGTTATGTTTTTTTTTTTTT10.6993006993006993No Hit
TCTCCCATGTCAGATCGGAAGAGCACACGTCTCCCATGTCAGATCGGAAG10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGATCGTCGAGACCACCGTCGCGGTCTGGCCCTTGGCGAGGAAATCGAG10.6993006993006993No Hit
CCCGGTGTTCGAATTGTAGTCTGAAGAAGCGTCCTCAGCGGCGGACCGGG10.6993006993006993No Hit
CGTCTCCCAGTGTAGATCGGAAGAGCACACGTCTCCCAGTGTAGATCGGA10.6993006993006993Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCACTTCCGAGACGTGTGCACTTCCCAAACGAGAGCTC10.6993006993006993No Hit
GGGTTAGCGTAAGCGAAGCTCTTGATCGAAGCCCCAGTAAACGGCGGCCG10.6993006993006993No Hit
GGCCCACCTGAATAGAGGCATTTTCTTCTTTCCTGAAGAGGGGGTTAAAA10.6993006993006993No Hit
CTTCCGATCAGACGTGTGCTCTTCCGATCAGACGTGTGCTCTTCCGATCA10.6993006993006993TruSeq Adapter, Index 3 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers