FastQCFastQC Report
Wed 29 Mar 2023
HW7FWDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7FWDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858973574
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGCCT1042859535.609867426765261No Hit
TGTGCGGG881507574.741904792671356No Hit
ACCATAAC706167073.798693428871733No Hit
CAGCCCAC698609943.758041264119659No Hit
TTCCCGAC691878043.72182826951794No Hit
GGCAATGG664913523.5767776868892702No Hit
CAGAGGCC634342163.412324784343599No Hit
AGGCCCGA616061793.3139889593718346No Hit
CGATCACC613342303.299359972504913No Hit
TTTGGGTA601692583.236692486732466No Hit
ACGGACAT557319012.9979931818008727No Hit
GCAGTAGA556905642.99576953534467No Hit
TCAGCCGT549460432.9557194232606125No Hit
TAATGGGA541628262.9135877323665493No Hit
ACATTACT525181722.8251166522499473No Hit
TCAAGGCC524206882.8198726831390664No Hit
CAGTACTG520483132.7998414677840926No Hit
AGTAGTCT510003602.743468799842122No Hit
GATGCCAA501621682.6983798318372436No Hit
AGCATCCG495813732.667137053127362No Hit
CCTCATTC493635122.655417628868349ABI Solid3 Adapter A (100% over 8bp)
TTCAGGTG492229322.647855391192344No Hit
CCAAGATG489596142.633690692797336No Hit
GTTTATCT454064172.442553118294085No Hit
GGTCAATA446819092.40357956804393No Hit
ATCTTTAG445893342.398599669389383No Hit
CTCCTAGT427985372.302267100435931No Hit
TACGTGAC412124342.2169456616493033RNA PCR Primer, Index 17 (100% over 8bp)
GTGGCAAT402002932.162499433141485No Hit
AGGTATTG397072402.1359765709074034No Hit
GATCTTGA390644612.10139947906543No Hit
GGGGGGGG363034991.9528787018679803No Hit
GTGTATTA117013490.6294521430351435No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)