FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_SG91_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_SG91_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8055103
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132490.16447958517724728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC7302.2191671E-109.58969156
CGCCGTA6553.4571713E-89.0841959
GTGTGCC23950.06.57619764
TGGTCGC10309.7667E-76.45678855
CCCGCGT6950.00314080586.04211240
GCCTCTA26200.05.878187768
GCCGTAT10556.283242E-55.63991360
CCGTATC13051.5630503E-65.632215561
GTGCCTC25700.05.583862366
TGTGCCG8150.00297771025.58316670
GTCGCCG15101.2586315E-75.563256357
AGTGTGC28950.05.440208463
CGTATCA16002.3141802E-65.031028762
Produced by FastQC (version 0.11.5)