FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_SG162_col_B_l2-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_SG162_col_B_l2-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5677383
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72670.12799911508524262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2505.254429E-614.0045396
GGGAGCT6900.0028903326.08710589
ACTCCCT7650.009094495.489508630
TAGCAGG10100.00121461535.197735363
Produced by FastQC (version 0.11.5)