FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_SG141_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_SG141_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587161
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32240.12461536023463558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGT3851.8620995E-812.7296687
CGGACTG3651.2754936E-712.4680996
GACTGTA5251.2557939E-79.9999488
CAGTCCG3450.00160151229.12968127
ACTGTAG5201.2032933E-58.7497859
ACAGTCC4753.833681E-48.10459426
AGTCCGG4200.0094452457.498945740
TACAGTC4750.00342377767.367527525
GTAGGAT15553.6379788E-126.75289870
CGGCTTC5250.0090332266.666631747
AGTGTAG18000.06.6114667
GGGTGTA5850.0034964456.581670869
GAGTGTA18305.456968E-126.12042366
GGATAGG8801.888688E-45.966339670
TGTAGGA19555.456968E-125.908354869
AGAGTGT22005.456968E-125.568260765
TAGGATA20353.603418E-95.16007770
GTGTAGG39250.05.08316968
Produced by FastQC (version 0.11.5)