FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_SG138_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_SG138_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608396
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23390.14542438553689516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG1107.483366E-419.08921212
CTAGCAG2150.00477551311.39610860
ACCTCGG2200.00566787411.13710659
CGAACCC3900.0048517188.07746167
AGTCATG4200.0094213237.50073369
CAGTTGA6050.0049796626.362279441
TGTTCAG7250.00500817045.793489568
GTAGGGA11152.3949036E-55.65060168
ATGAGAG8700.0063860925.23234943
TAGGGAA13551.7808903E-55.16655769
Produced by FastQC (version 0.11.5)