FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_SG138_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_SG138_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122198
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25970.1223731244681222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGG1950.00230137612.56410761
TTCGCGG2200.005676666711.13505518
GGGTGTA5151.0199243E-48.15591969
GATAGGG6907.360959E-78.11651870
TAGGGTG4905.343159E-47.85770167
GGATAGG7401.7141389E-57.095098570
GTGTAGG30600.06.977619670
AGGGTGT5800.00319502486.63840368
CGTGTAG23500.06.4047169
ATAGGGT7358.646275E-46.190915670
GTCGTGT25600.05.742595767
GCGTCGT26850.05.605480765
CGTCGTG26850.05.4752566
GTAGGAT11604.50375E-55.431420370
TCGTGTA27250.05.39487968
AGCGTCG28000.05.250001464
GAGCGTC28850.05.216270463
AGGATAG9500.00318664555.15753263
GGGAAAG22201.20599E-95.045403567
AGTGTAG13256.772746E-55.01922469
Produced by FastQC (version 0.11.5)