FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_SG137_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_SG137_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3770544
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56990.15114529892768788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2405.0386006E-1018.95921556
CGCCGTA2852.0330754E-512.28094659
TGGTCGC3601.4611123E-611.66751855
TCGGTGG5906.533577E-68.3050351
CCGTATC4500.00201403357.777622761
CTCGGTG5753.6542054E-47.30439650
GCCGTAT4850.0041917527.21644760
AGGGTGT9351.8234459E-77.11225269
GTCGCCG5550.00201156926.93725957
GGGTGTA7158.3689214E-56.853101370
ATCTCGG7904.2804673E-56.645348548
TAGGGTG8551.8153672E-56.54966468
GGTGGTC7501.561305E-46.533463553
GGGGGAG6700.0020802586.26811621
GGATAGG11901.8282335E-65.88231469
GATAGGG11355.1284496E-65.85899270
GGGGGGA7250.00502086765.792680720
CGGTGGT9102.9854866E-45.76926952
TCTCGGT9150.0019904225.35522749
GTAGGAT19453.483001E-85.03852769
Produced by FastQC (version 0.11.5)