FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_NAC_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_NAC_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761744
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51470.1368248344384945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC3802.8004379E-611.05300356
CGCCGTA4156.757655E-710.96407959
TGGTCGC4451.6575541E-610.22519955
GCCGTAT5251.3480967E-69.33352360
GCTATAG14050.08.47071169
CCGGGCT3850.0043340068.17991739
CGTATCA7907.011058E-77.53159962
CCGTATC6301.3201335E-47.22236961
GGCTATA16800.06.87568868
TCGGTGG7702.1968347E-46.3638551
AGGCTAT18800.06.330505470
AGTGTAG31550.06.213066670
TGTAGGC19900.06.15647770
CTATAGT19203.6379788E-126.01630870
GTAGGCT18203.274181E-115.96221569
GTCGCCG7950.00221652145.72346357
TAGGCTA19805.4569682E-115.657207569
TGGGGGG11152.4037923E-55.650038258
GGTGGTC8800.00121656855.56822153
GTGGTCG10152.185574E-45.5172854
Produced by FastQC (version 0.11.5)