FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_HDL_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_HDL_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11736446
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182240.1552769893032354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC7402.8721843E-98.987164556
TCGCGAC4753.9655923E-58.8428155
CGCGGGC5109.121854E-58.23528464
CTCGCGA6856.6117354E-78.17531254
GCGGGCG5301.4262563E-47.924822365
CGCCGTA7301.7277489E-67.671582759
CGTCGCG6501.9406312E-47.000140761
GCGTCGG7158.363952E-56.85378170
GTCGCGG6259.4824744E-46.71973462
GGGCGGC7351.200347E-46.66767
CGGACGC5900.00382742686.525499345
TGGTCGC10258.98297E-76.488322355
CGCGACG7055.2286405E-46.454387756
GGAAAGG84200.06.44859271
ACGTCGC8752.5503296E-56.40018360
GGAAGGT45300.06.1863871
GAAAGTC68250.06.15788132
AGCCGGG8003.6015827E-46.12522765
GCGGCGA10407.8263165E-66.058253369
TCTCGCG8801.89268E-45.965978653
Produced by FastQC (version 0.11.5)