FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_HDL_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_HDL_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11761639
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184230.15663633274240094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGG4501.6322701E-710.88878662
GCGCGCC2953.8047327E-410.6783251
CGCCGTA6754.9112714E-1110.37119959
CGCGGGC4301.2249588E-59.76776564
TCGCGAC5057.937997E-79.70391255
CTCGCGA6503.052628E-89.15418854
GGTCGCC6755.6241333E-88.81566956
CGGACTG7301.9799336E-78.153216
TCGCGGG7352.2208042E-78.09502463
GGGCGGC6101.021079E-58.03339567
GCGGGCG5703.8113394E-57.982958365
TGTGCCG8604.191861E-87.73337470
CACGGTC14650.07.64553266
CGCGACG5753.652376E-47.305055656
CGTCGCG6056.541984E-46.94246761
CTCACGG19700.06.929163564
CGGGCGG8351.2805813E-56.707008466
TGGTCGC9955.3761687E-76.684064455
GCCGTAT11158.204552E-86.59239960
GCGTCGG6000.00455761376.41734370
Produced by FastQC (version 0.11.5)