FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_HALO-bZIP53_eGST-bZIP63_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_HALO-bZIP53_eGST-bZIP63_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190339
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4700.24692784978380677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCC200.00694657152.49349619
GTTGAGC450.004285543731.1318021
ACAGGCA500.007181382328.0112532
TACGTAC800.002660967221.8722957
CAACCAT950.007183289618.42361364
GATTAGT2405.5088603E-513.12682369
AGATTAG2550.001346279610.98191868
AAGATTA3004.396101E-410.50145970
TAAGATT3800.00380522528.29062669
AGTGTTA5754.1691677E-57.91414370
GAAAGAG9352.6520866E-97.86205569
GTTAAGA4600.00246885587.609752764
GAGTGTT6351.4283188E-47.166349469
GGGAAAG9805.2728865E-87.141972567
AAAGAGT9452.113502E-77.03801570
TGTTAAG5000.00557041177.00097368
AGAGTGT6501.9037588E-47.000972768
GGAAAGA9955.180991E-76.68434668
AAGAGTG6954.3303304E-46.54595267
AGGGAAA11103.4047134E-65.990248766
Produced by FastQC (version 0.11.5)