FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_HALO-bZIP53_eGST-bZIP63_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_HALO-bZIP53_eGST-bZIP63_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATATAGATATAAAAGGAAAAGACAAAAAAAAAATAAAATACAAAA12.127659574468085No Hit
TTATTGTCTTTAAAGTATTGAAAAATTATTGTTATCTAACTCATACAAAC12.127659574468085No Hit
ATGTCCATATTCATTCACAAAACGTATGAGTAAATCGCGCCTGAGGTGGA12.127659574468085No Hit
AAAAGTATCTTCATTCTCATATCTGTTGTACGCGTGTTCTGAGGTGTGGA12.127659574468085No Hit
TGAAAATAAAAAAAATAATAATCAAAATTAAACAAAAATTAAAATAAATT12.127659574468085No Hit
AAAACCTGGTATGAGTTTTCTTGTCAAATGAAATTTGATTCTCCATGAAT12.127659574468085No Hit
CTTCGCTTACATTACTTATATAAAACCCTCTCTCGCATCCTTCCTCAGTT12.127659574468085No Hit
AATTCCTGACATAGAAGTACGACCGAGACAAAGAAGAATAAAGTGTACGA12.127659574468085No Hit
CTCAGAGTTTTGATGCGTTCCAATTCTCGATTACATGCGTCTTGCATTCT12.127659574468085No Hit
GTCAATGCTCGTGAAGGCTTGATGGGGTTTCCGTGCATGGCGGGTGGGGC12.127659574468085No Hit
GGGACAAAGCTTCTACGACAGCTTCCCCCTCATGTAGTCGTCGGCCTTCC12.127659574468085No Hit
ATGATTAGAATAGTTTCAGAACTAATACTTGCATTTTTGTATATGAATTT12.127659574468085No Hit
CGAAAAATCCCGCGCGTGACCATATTGCTTTGGTTCTCTTGTACCCATAT12.127659574468085No Hit
AATTGTTTTATTATTAAAATAAAAAAGAATGCACAGATATATATGTCTCT12.127659574468085No Hit
TGTGAATTTTCTCCAAGTGTCAAACTTCCATATGCTCATTAACAATCCAA12.127659574468085No Hit
CGTCTGGGACGGTGGGACGGGACTCCAAAGGCGTGGCAAGTCGCGGCGCG12.127659574468085No Hit
CTCGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAGATACAGAGCC12.127659574468085No Hit
ATGTTTAAAAAAATTGCCTGTCCTAAAATAGTAAATGATTAAGAAATAAA12.127659574468085No Hit
CTCGGTAGTTAACATTTTCGACCGACGTTAAACATTTTTGAGTGATACGA12.127659574468085No Hit
ATCCTTTGCTGATTATTAGGAGCCAGATTAATATCTTTGACAGTCCGGGA12.127659574468085No Hit
CTAGATCTACTTTTTGGTGTTTTTGATGAGAGAGAAAAGAGAAAATGTCT12.127659574468085No Hit
TTCTTTCAATTGAAAGTCTTTACCGCCGAATCTTTAATATTTACATACGC12.127659574468085No Hit
TTAGACAATAATTCATAAACATATCATAAATAAAATCAACATTAATAAAA12.127659574468085No Hit
AATAAAAATCTTTTAAAACATTGTAAATTATTTTAAAACCTTTATAAATA12.127659574468085No Hit
ATATATAGCGCCAGATTTATTTCACAAAACTATTGTGCGAAATATATCAT12.127659574468085No Hit
CCAACCTTCTTCTAGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGTCCGT12.127659574468085No Hit
GTCACACCCCACGCGACTCACAATGGGATAATCTGAGTTACAAGAACAGA12.127659574468085No Hit
TAATAAGTTGATTCGAGATTTCGGTATCAACCTCTTGGCCTAAAAAAAAT12.127659574468085No Hit
AAGAGCAAAAAGGGAATGAACAAAGTAAAACAAATTGAGGAATGGAAGAG12.127659574468085No Hit
ATTTTCAATTTAATACAAAATATCAAAAGATACGTAGTGTCAATAATTGA12.127659574468085No Hit
ATAAGTTTTCAATCAATATTTAACGTAATTATTTTATAAATTAACATCGA12.127659574468085No Hit
ATCCTTTAGATTCAACGTCAACTATGCTACAACCTCGAATCGTTGGCGAG12.127659574468085No Hit
ATTCTTGTGATCGTTTTTGAGGTCGGCAAAATAGTTCTTGTGACCTAATA12.127659574468085No Hit
AATGACATTTAGAACAAAACTAATGTTACATAATGTGGTTTCGGGTCTGG12.127659574468085No Hit
AAAAGATATGATCAGATCAGACACCAGTGAACCCCCTTATCCCCAGCTGC12.127659574468085No Hit
TCTTTAATTGCAGAGATGTACCTTCTTTAACTCTGTTTTAGGTAGCTGAG12.127659574468085No Hit
CTCTCTGTCATTTATGCAAAAAAAAAAAAATGGTTATGTAGACAGCTAAT12.127659574468085No Hit
ATTACAAAAAGAATCAACCAAAAATATCATCTAAAAAATAAAATAATCCA12.127659574468085No Hit
TAGTGTTTTGGAGTCGAATATGACTTGATGTCATGTGTATGATTGAGTAT12.127659574468085No Hit
AATATAGTTAACTAAAAAACTAAAATAAAATAATTGGCCTAAATAAAATG12.127659574468085No Hit
TACCTTGGGAATTTGGCGCCCAAAATGTCTTTCTGGCCATATTTTCTCCA12.127659574468085No Hit
ATCGACCCAGGAAGAATATATTCTAACTTTATGGATAATCCATGATCAAC12.127659574468085No Hit
AATCATTTATCTGTTAAAAGTTCATAAAATAGCCTCATCATATGAGATTT12.127659574468085No Hit
CTAATCTTGTTAGTATCAATGATATACGAATCATATTTAAACTGCTAGTT12.127659574468085No Hit
AAACTGTAATAAGATAATCGTTGACTCTAGCGAGAGATCTCTGTAATGAA12.127659574468085No Hit
TTTTAGAAAGTAATAAAAGAGAAAAGCAAGCTAAATAATCCATCCTAAAA12.127659574468085No Hit
CTAACCAGTACTACCCCACACCACAAAGCCATCTAAACTCGCCACCCTAT12.127659574468085No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)