FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_HALO-CCA1_eGST-LHY1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_HALO-CCA1_eGST-LHY1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779396
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52050.137720418818245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGC20700.06.76377169
GGGGGAT5850.0035028316.580711463
GCTATAG15502.0008883E-116.548857770
GTAGGCT19250.06.36409470
TAGGCTA19050.06.247167670
GGCTATA19100.06.23081469
AGGCTAT18551.8189894E-126.2267868
TGGGGGG11357.839426E-76.168007457
AGCACGC6300.0075701446.111388766
AGTGTAG31550.05.99076367
GTGGGGG11152.3982571E-55.65085556
GGGGGGA10707.78525E-55.560411562
Produced by FastQC (version 0.11.5)