FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DUO3_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DUO3_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220349
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17950.14708907042165809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCG901.929242E-423.33284248
CGGCACT1150.001008764118.25898663
GTGCGTT1554.0547852E-415.806766516
CGCCTGT2550.001356734410.98061169
AAGTACG2550.001357663710.97971133
CACCGGT2700.00218563510.36972727
GCCTGTG3055.168394E-410.32807470
TCGGTTG3055.168394E-410.3280749
TCGCCTG2800.00295459719.99978968
CTTCGCC5109.0990376E-58.23545869
TTCGCCT4854.7848816E-47.938302570
TCTTCGC6955.755099E-57.05021168
GTCTTCG7156.1944715E-46.36350267
GAGTGTC8953.5417554E-56.256594765
AGTGTCT9851.4195971E-45.68492664
GATATGA8700.00105177455.631603230
AGAGTGT12602.9466022E-55.27788370
Produced by FastQC (version 0.11.5)