FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DUO1_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DUO1_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2690583
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28000.10406666510566669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCCC3600.002342318.755832
AACTGGC6958.8872184E-88.5686091
AACGACC6755.212896E-78.3035231
AACTCCC8553.7225618E-87.78455351
AACCTAC9301.6337799E-77.15676741
ACGACCT4900.0046006877.1476172
AACCGTG9351.7943239E-77.11849551
AACGGTC7652.6996551E-56.86872341
AACCGGG7209.060057E-56.8114841
AACTGTG10451.7385173E-76.70441
AACGACA8954.926904E-66.65383631
GAGTGTA12251.0522854E-86.57189570
ACTGGCT6400.00122991396.56687262
AACTACC12904.3874024E-96.51730061
AACTGAC10351.0453168E-66.43071841
GTAGGCT8257.787015E-56.364087670
AACTATA35400.06.234241
AACCATC24500.06.14820861
AACCTCT17154.1836756E-116.1277831
AACTAAT41600.05.97876171
Produced by FastQC (version 0.11.5)