FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DUO1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DUO1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997689
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12490.12518931250119025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC808.662445E-526.2531669
TCCCCCG952.7787845E-422.10792570
TGGACGG1300.002291738816.15255244
ACAGGAC1400.003742950715.006323
CGTCCCC1450.004735531314.48450368
CAGAACG1450.004745002414.480144528
CGTACTG2050.003341616411.95325856
TCCGTCC2500.001148552411.20134766
CTATTCC3550.00207557168.87208324
ACATCAC3650.0026705448.62901227
GGAATTA4653.0304043E-48.2822285
GATCCGT4750.00340959487.3696776
AGGGATT5000.0056209987.00084270
TACGGAA6702.7858705E-46.79492952
TTTAAGC5250.0090239826.66646612
GTAGGGA7401.305461E-46.622418466
TGTAGGG7903.0485675E-46.20327865
TGGTGAT6250.00695605866.160432352
CGTGTAG8406.712355E-45.833450363
GTGTAGG8650.00599017375.260484764
Produced by FastQC (version 0.11.5)