FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DAZ2_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DAZ2_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9950432
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201020.20202137957427377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA11000.015.273910559
GGTCGCC11700.014.95857556
GCCGTAT13350.013.37175660
TGGTCGC13150.012.77678555
CCGTATC16350.011.13224961
TCGGTGG20550.08.51617551
GTCGCCG23850.08.36551257
CGTATCA21800.08.18804962
TCGCCGT21800.08.02786358
GTGGTCG24750.07.77787254
GGTGGTC24900.07.59045353
AGGCTAT61650.07.437871569
CTCGGTG21000.07.333827550
GGCTATA62450.07.11835370
TAGGCTA68200.06.723327668
GTAGGCT70300.06.57224567
CGGTGGT29300.06.450687452
ATCTCGG27750.06.30666848
AGTGTAG119050.06.29205670
TGTAGGC77600.06.269626666
Produced by FastQC (version 0.11.5)