FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DAZ2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DAZ2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341502
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46100.1379619105420257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATA17600.011.3437372
TTACCAT24900.010.973731
GGCCCAC3401.2287514E-410.29305412
GCCCACT3853.90255E-59.99896713
GACGGCT3753.299629E-49.33139140
CCGTGTG3400.00140355989.26291734
GTGCCCT3150.0077991158.88970170
TTACCAA30400.08.52741051
CGTCCTA4250.00114552758.2354364
GCCGACA4703.418634E-48.19150257
TGACGGC4804.2979498E-48.01904539
CGGCTCA4450.00180518677.86459142
GCAGCCG4550.00224404747.692434354
TACCAAA36350.07.61231332
TTCCGTG6159.8216486E-57.397277432
GTCCTAC4750.00341930337.36865365
GTGCCTC11958.54925E-107.02993170
TTACCTA15700.06.91705271
TTTCCAT43750.06.8861961
GCTCTCG5800.00320172036.63734524
Produced by FastQC (version 0.11.5)